Service

Metabolic Pathway Analysis for Bacterial Genomes

Reconstruction of metabolic and regulatory pathways.

Pathway analysis puts annotated genes together into complete metabolic routes. It shows which pathways your strain has in full, which are missing steps, and what that means for its growth and products.

It is especially useful for questions like whether a strain can make a vitamin, ferment a sugar or produce a metabolite of interest.

What you receive

  • List of KEGG pathways and modules with completeness
  • Pathway maps with your strain's genes highlighted
  • Missing steps in pathways of interest
  • Interpretation of key metabolic capabilities

How we do it

  1. 1

    KEGG mapping

    Genes are assigned KEGG orthology numbers with BlastKOALA.

  2. 2

    Pathway reconstruction

    Assigned genes are mapped onto KEGG pathways and modules.

  3. 3

    Gap check

    We list missing steps and consider alternative enzymes.

  4. 4

    Interpretation

    We explain what the pathways mean for your research question.

When you need it

  • Predicting vitamin, amino acid or metabolite production
  • Explaining growth requirements
  • Choosing strains for metabolic engineering

Questions

Does a complete pathway mean the product is made?

It means the genes are present. Expression and activity depend on conditions, so lab measurements confirm production.

Further reading

For Research Use Only. BacSeq Analytics provides bioinformatics analysis and interpretation services for microbial genomic research. The reports are intended for research and investigational purposes only and are not intended for clinical diagnosis.

Ready to turn your genome data into insight?

Get a highlight report within 72 hours. Share your project and our bioinformatics team will respond within one business day.