Understanding ANI: How Average Nucleotide Identity Defines Species
A practical guide to interpreting ANI thresholds and why 95% matters for microbial species delineation.
18 June 20266 min read
Microbial WGS Bioinformatics — Analysis OnlyAdvanced Bioinformatics.
Advanced Bioinformatics.
Actionable Insights.
Within 72 Hours.
Precision Genomics. Reliable Insights. You upload your sequencing data — FASTQ, FASTA, assembled genomes or contigs — and we deliver advanced computational analysis with publication-quality reports and clear scientific interpretation.
72-Hour Highlight Report
Publication-Ready
Confidential & Secure
Secure Upload
Drop your genome data
FASTQFASTAAssembled genomesContigs
1Quality Control
2Assembly & Annotation
3Functional Analysis
0h
Highlight report turnaround
0+
Analysis modules
0
Best-in-class tools
0%
Confidentiality assured
Why BacSeq
Everything you need from a genomics analysis partner
Specialist microbial WGS bioinformatics, delivered fast, affordably and with rigorous scientific interpretation.
Highlight Report in 72 Hours
Fast-tracked key findings delivered within three days of data upload.
Affordable Pricing
Transparent, research-friendly pricing with no hidden costs.
Publication-Ready Reports
Journal-grade figures, tables and interpretation, ready to submit.
Scientific Interpretation
Every result is contextualised by an expert bioinformatics team.
Research Support
Guidance from raw reads to manuscript, tailored to your study.
Secure Data Handling
Encrypted transfer and storage across the full analysis pipeline.
Confidentiality Assured
Your data and findings remain strictly private to your project.
Expert Bioinformatics Team
Specialists in microbial genomics using state-of-the-art tools.
Analysis Only
We analyse — you sequence elsewhere
BacSeq Analytics is a pure bioinformatics service. You provide the sequencing data; we turn it into insight. That focus is exactly why our analysis is fast, affordable and deep.
What we provide
Advanced computational analysis of microbial whole genome sequencing data, from quality control and assembly through functional bioinformatics, interpretation and publication-quality reporting.
You upload
FASTQFASTAAssembled genomesContigs
What we do not provide
- DNA extraction
- Library preparation
- Sequencing
- Wet lab services
- Clinical diagnostics
Services
All services Comprehensive microbial genome analysis
Seventeen analysis modules spanning assembly, annotation, resistance, virulence, metabolites and comparative genomics.
Whole Genome Analysis
End-to-end interpretation of complete microbial genomes.
Genome Assembly
De novo assembly of reads into high-quality contigs and scaffolds.
Genome Annotation
Structural and functional annotation of genes and features.
Species Identification
Accurate taxonomic placement from genomic signatures.
ANI Analysis
Average Nucleotide Identity for precise species delineation.
AMR Analysis
Detection of antimicrobial resistance genes and determinants.
Why Choose Us
A specialist partner, built around your research
We focus exclusively on microbial WGS bioinformatics — so every project benefits from deep expertise, reproducible pipelines and genuine scientific interpretation.
- Fast turnaround
- Affordable services
- Publication-ready reports
- State-of-the-art bioinformatics tools
- Scientific interpretation
- Research-focused
- Confidentiality guaranteed
- Dedicated support
- No hidden costs
WGS Analysis Report
Escherichia coli — Isolate BSQ-0142
5.24 Mb
Genome Size
50.8%
GC Content
4,912
CDS
17
AMR Genes
AMR — Resistance by Antibiotic Class
Beta-lactam
Aminoglycoside
Tetracycline
Fluoroquinolone
Macrolide
99%Completeness
2%Contamination
94%ANI Match
Detected Features
3 Plasmids12 Virulence Factors6 BGCs (antiSMASH)88 CAZymes5 MGEs142 KEGG Pathways
Packages
Choose the depth your research needs
Three clear tiers — Basic, Standard and Premium — each building on the last. Contact us for tailored pricing.
Basic
Essential genome characterisation
Species-level identification and core quality assessment.
- Species identification
- Assembly QC
- Genome annotation
- Basic report
Most Popular
Standard
Comprehensive functional profiling
Everything in Basic, plus deeper functional insight.
- Everything in Basic
- AMR analysis
- Virulence analysis
- Plasmid analysis
- Mobile genetic elements
- Functional annotation
- Comparative genomics
Premium
Publication-grade deep genomics
Everything in Standard, plus advanced discovery and consultation.
- Everything in Standard
- CAZyme analysis
- Secondary metabolite analysis
- antiSMASH
- Pathway analysis
- Genome interpretation
- Publication-ready figures
- Consultation
Workflow
From upload to secure report delivery
A transparent, reproducible pipeline — no sequencing, pure analysis.
1
Client Uploads Data
Securely upload FASTQ, FASTA, assembled genomes or contigs.
2
Quality Control
Read quality assessment and trimming with fastp.
3
Genome Assembly / Validation
De novo assembly with SPAdes and assembly validation.
4
Genome Annotation
Structural and functional annotation with Prokka and RAST.
5
Functional Bioinformatics Analysis
AMR, virulence, plasmids, CAZymes and metabolite discovery.
6
Scientific Interpretation
Expert contextualisation of every genomic finding.
7
Professional PDF Report
Publication-quality report with dashboards and figures.
8
Secure Report Delivery
Encrypted delivery of your final report and data.
Technology Stack
Powered by best-in-class open bioinformatics tools
We build reproducible, Snakemake-orchestrated pipelines around the tools trusted by the microbial genomics community.
fa
fastp
Read QC & trimming
SP
SPAdes
Genome assembly
Pr
Prokka
Rapid annotation
RA
RAST
Subsystem annotation
Bl
BlastKOALA
KEGG functional mapping
an
antiSMASH
Secondary metabolites
db
dbCAN2
CAZyme annotation
Re
ResFinder
AMR detection
Pa
PathogenFinder2
Pathogenicity prediction
Fa
FastANI
ANI computation
Sn
Snakemake
Reproducible pipelines
fa
fastp
Read QC & trimming
SP
SPAdes
Genome assembly
Pr
Prokka
Rapid annotation
RA
RAST
Subsystem annotation
Bl
BlastKOALA
KEGG functional mapping
an
antiSMASH
Secondary metabolites
db
dbCAN2
CAZyme annotation
Re
ResFinder
AMR detection
Pa
PathogenFinder2
Pathogenicity prediction
Fa
FastANI
ANI computation
Sn
Snakemake
Reproducible pipelines
Applications
Genomics that serves every microbial research field
Microbiome Research
Probiotic Research
Food Microbiology
Industrial Biotechnology
Agricultural Microbiology
Environmental Microbiology
Academic Research
Biotech Companies
Pharmaceutical Research
Hospital Research Laboratories
Testimonials
Trusted by researchers and biotech teams
What our clients say about turnaround, clarity and publication-readiness.
“The 72-hour highlight report gave us the resistance profile we needed before our grant deadline. The interpretation was clear and genuinely useful.”
Dr. A. Sharma
Microbiology Research Lead
“Publication-ready figures straight out of the box. Two of the circular genome maps went directly into our paper with no reformatting.”
Dr. M. Nair
Principal Investigator, Probiotics
“Affordable, fast and confidential. BacSeq handled a batch of 40 isolates and delivered consistent, well-documented comparative genomics.”
R. Baruah
Biotech R&D Manager
Insights
From the BacSeq blog
Practical microbial genomics — methods, interpretation and best practice.
AMR Surveillance with WGS: From Reads to Resistance Profiles
How whole genome sequencing accelerates antimicrobial resistance detection and what a robust pipeline looks like.
30 May 20268 min read
Mining Microbial Genomes for Novel Natural Products with antiSMASH
Biosynthetic gene clusters hold the key to new metabolites. Here is how we screen genomes for discovery.
12 May 20267 min read
Ready to turn your genome data into insight?
Get a highlight report within 72 hours. Share your project and our bioinformatics team will respond within one business day.